Study on the Cupric Phenanthroline-Induced Serotonin Release in Digitonin-Permeabilized Platelets

概要

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Digitonin-permeabilized rabbit platelets released serotonin dose-dependently in response to cupric phenanthroline, which is a mild oxidant which catalyzes the formation of disulfide bridges. The serotonin release induced by cupric phenathroline was inhibited by ethylene glycol bis(β-aminoethylether)N, N'-tetraacetic acid (EGTA), but EGTA did not restore the decrease in proteinbound free sulfhydryls of permeable platelets. An alteration in calcium movements was suggested to be implicated in the serotonin release from cupric phenanthroline-treated permeable platelets.The agent suppressed the calcium uptake to calcium-storage sites, probably the dense tubular system, and enhanced the calcium efflux. Moreover, it tended to inhibit Ca^<2+> -adenosine triphosphatase of permeable platelets. On the other hand, a sulfhydryl modifier, chloromercuriphenylsulfonic acid, also decreased the amount of free sulfhydryls in protein and released serotonin in permeable platelets, but EGTA did not inhibit either reation. Neither cupric phenanthroline nor chloromercuriphenylsulfonic acid elicited serotonin release from intact platelets. It is suggested that both agents act on intra-platelet target molecules to exert their effects, but the mechanisms involved are different.
公益社団法人日本薬学会の論文
1988-02-25