Chromogranin A-Like Proteins in the Heat-Stable Fraction of Sea Urchin Eggs, Embryos and the Substances Secreted with Sperm(Developmental Biology)

概要

論文の詳細を見る
Chromogranin A, an acidic, sulfated and heat-stable glycoprotein, is a major protein component in a variety of secretory granules of neuron and para-neuron cells. Widely phylogenetic occurrence of this protein from protozoa to mammals has been reported. In the present study, several immunoreactive proteins against bovine adrenal chromogranin A anti-serum were detected by immunoblotting of the heat-stable fraction obtained from sea urchin unfertilized eggs. Apparent molecular weights of the major components were about 166, 112, 105, 36 and 34 kDa, respectively. Content of the proteins was significantly decreased after fertilization, suggesting that these are released or decomposed at fertilization. At the pluteus stage, at which nervous system appears, the content of the immunoreactive proteins was markedly increased. Apparent molecular weights of the major component were about 214, 105, 89, 84, 36 and 34 kDa. No immunoreactive band was detected in the heat-stable fraction obtained from "washed" sperm, while the fraction from "dry" sperm contained several immunoreactive proteins. This suggests that not sperm but substances secreted with sperm contain these proteins. Although the role of the immunoreactive proteins on fertilization, embryonic development and sperm functions is unknown at present, they may take a part of the role on these processes.
社団法人日本動物学会の論文
1992-04-15