Degeneration of Respiratory System in Sea Urchin Spermatozoa during Incubation in Seawater for Long Duration
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概要
- 論文の詳細を見る
Motility and respiration were examined in spermatozoa of the sea urchin Hemicentrotus pulcherrimus after dilution and incubation in seawater at pH 8.2 at 20℃. Almost all spermatozoa were motile during 12 hr of incubation, but their respiratory rate decreased gradually. The acrosome reaction was also induced by egg jelly solution during 12 hr of incubation in seawater. However, the ratio of spontaneous acrosome reacted spermatozoa was quite low during the same period. An intracellular pH (pHi) of spermatozoa was about 7.5 just after dilution in seawater and was almost constant during 12 hr of incubation. Upon dilution and incubation in seawater, activity of NADH-cytochrome c reductase decreased in propotion to the decrease in the respiration in spermatozoa, whereas cytochrome c oxidase activity was hardly changed. These suggest that the degeneration of respiratory system during 12 hr of incubation in seawater is due to the decrease in the NADH-cytochrome c reductase activity. In energy production system, phosphatidylcholine as a preferred substrate was efficiently hydrolyzed during 4 hr of incubation and then the activity of the energy metabolism decreased gradually. Beyond 12 hr incubation in seawater, the number of immotile spermatozoa increased and respiratory rate declined rapidly. Also, the percentage of the acrosome reaction induced by the egg jelly solution decreased. These are probably due to the increase in the number of dead spermatozoa after 12 hr of incubation in seawater. It is thus concluded that the life-span of H. pulcherrimus spermatozoa is about 12 hr after dilution in seawater.
- 社団法人日本動物学会の論文
- 1996-12-15
著者
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Fujiwara Akiko
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima Uni
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Fujiwara A
Kanagawa Univ. Kanagawa Jpn
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Fujiwara A
Department Of Biology School Of Education Waseda University
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Fujiwara Akiko
Department Of Applied Microbiology Nippon Roche Research Center
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Yasumasu Ikuo
Department of Biology, School of Education, Waseda University
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Mita Masatoshi
Department of Biosciences, School of Science and Engineering, Teikyo University
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Yasumasu I
Department Of Biology School Of Education Waseda University
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Yasumasu Ikuo
Department Of Biology Colege Of General Education University Of Tokyo
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Mita Masatoshi
Department Of Applied Physics Faculty Of Engineering Osaka City University
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Tazawa Eigoro
Biological Institute, Faculty of Science, Yokohama City University
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Tazawa E
Kanagawa Univ. Kanagawa Jpn
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Tazawa Eigoro
Biological Institute Faculty Of Literature And Science Yokohama City University
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Ohtake Tateru
Department of Biology School of Education, Waseda University
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Ohtake Tateru
Department Of Biology School Of Education Waseda University
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