cDNA Cloning of Na^+, K^+-ATPase α-Subunit from Embryos of the Sea Urchin, Hemicentrotus pulcherrimus
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概要
- 論文の詳細を見る
Na^+, K^+-ATPase α-subunit cDNA of the sea urchin, Hemicentrotus pulcherrimus, was obtained by twice screening prism and gastrula λgt10 cDNA libraries using an oligonucleotide probe derived from a mostly conserved region, FSBA (5'-p-(fluorosulfonyl)-benzoyladenosine) binding site of cation transport ATPases. The 5'-end of the non-coding region was determined by primer extension and the region was amplified by 5'-RACE method. The sea urchin α-subunit cDNA consists of 4401 nucleotides and encodes 1038 amino acid residues (MW, 114 kDa). The predicted primary structure, except N-terminal region, has similar degree of high homology to various metazoan Na^+, K^+-ATPase α-subunits. Alignment of amino acid sequence and a hydropathy profile also predicts eight putative transmembrane segments at least. The phylogenetic tree suspected from alignment of amino acid sequences of 21 species suggests that sea urchin and vertebrate Na^+, K^+-ATPase α-subunits seem to have evolved from a common origin, before vertebrate α-subunit divided into three isoforms.
- 社団法人日本動物学会の論文
- 1996-12-15
著者
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Akasaka Kouji
Mmbs Graduate School Of Science University Of Tokyo
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Akasaka K
Misaki Marine Biological Station Graduate School Of Science The University Of Tokyo
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Koji Akasaka
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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YAMAZAKI Ken
Department of Pathology, Keio University School of Medicine
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Mitsunaga-Nakatsubo Keiko
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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Shimada Hiraku
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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Akasaka Koji
Graduate Department of Mathematical & Life Sciences, Hiroshima Univ.
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Shimada Hiraku
Graduate Department of Mathematical & Life Sciences, Hiroshima Univ.
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Mitsunaga‐nakatsubo K
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Yasumasu Ikuo
Department of Biology, School of Education, Waseda University
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Mitsunaga-nakatsubo K.
Dept. Math. & Life Sci. Grad. Sch. Sci. Hiroshima Univ.
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Yasumasu I
Department Of Biology School Of Education Waseda University
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Akasaka Koji
Graduate Department Of Gene Science Faculty Of Science Hiroshima University
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Yamazaki K
Univ. Tsukuba Ibaraki Jpn
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Yasumasu Ikuo
Department Of Biology Colege Of General Education University Of Tokyo
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Shimada H
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Shimada Hiraku
Graduate Department Of Gene Science Faculty Of Science Hiroshima University
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Okamura Chisato
Department of Biology, School of Education, Waseda University
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Hatoh-Okazaki Midori
Department of Biology, School of Education, Waseda University
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Kawashita Hiroto
Department of Biology, School of Education, Waseda University
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Shimada H
Hiroshima Univ. Higashi‐hiroshima Jpn
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Okamura Chisato
Department Of Biology School Of Education Waseda University
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Shimada Hiraku
Graduate Department Of Mathematical & Life Sciences Hiroshima Univ.
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Yamazaki Ken
Department Of Electrical And Electronic Engineering College Of Industrial Technology Nihon Universit
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Kawashita Hiroto
Department Of Biology School Of Education Waseda University
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Mitsunaga Keiko
Graduate Department Of Mathematical & Life Sciences Hiroshima Univ.
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Yamazaki Ken
Department Of Biology School Of Education Waseda University
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Hatoh-okazaki Midori
Department Of Biology School Of Education Waseda University
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Akasaka Koji
Graduate Department Of Mathematical & Life Sciences Hiroshima Univ.
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