Cloning of Cyclin E cDNA of the Sea Urchin, Hemicentrotus pulcherrimus
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概要
- 論文の詳細を見る
cDNA encoding maternal cyclin E (HpCycE) has been cloned from the oocyte cDNA library of the sea urchin, Hemicentrotus pulcherrimus, by differential screening with a cDNA probe covering the total poly (A)^+ RNAs of 16 cell-stage embryos and gastrulae. In this communication we describe similarity of amino acid sequences between HpCycE and those lof cyclin E from other organisms and maternal origin of HpCycE. The amino acid sequence deduced from the nucleotide sequence of HpCycE cDNA is highly similar to those of human, rat, chicken, Xenopus, Zebrafish and Drosophila, while its similarity to other cyclins is much lower. A gene for HpCycE exists as a single copy in the genome of H. pulcherrimus. Northern blotting revealed that the mRNA for HpCycE is maintained at a high level up to the morula stage and thereafter declines.
- 社団法人日本動物学会の論文
著者
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Akasaka Koji
Misaki Marine Biological Station Graduate School Of Science The University Of Tokyo
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Akasaka Kouji
Mmbs Graduate School Of Science University Of Tokyo
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Akasaka K
Misaki Marine Biological Station Graduate School Of Science The University Of Tokyo
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Koji Akasaka
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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AKASAKA Koji
Laboratory of Molecular Genetics, Graduate Department of Gene Science, Faculty of Science, Hiroshima
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MITSUNAGA-NAKATSUBO Keiko
Laboratory of Molecular Genetics, Graduate Department of Gene Science, Faculty of Science, Hiroshima
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SHIMADA Hiraku
Laboratory of Molecular Genetics, Graduate Department of Gene Science, Faculty of Science, Hiroshima
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Mitsunaga-Nakatsubo Keiko
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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Shimada Hiraku
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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Kurokawa Daisuke
Laboratory of Molecular Genetics, Graduate Department of Gene Science, Faculty of Science, Hiroshima
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Mitsunaga‐nakatsubo K
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Kurokawa Daisuke
Laboratory Of Molecular Genetics Graduate Department Of Gene Science Faculty Of Science Hiroshima Un
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Mitsunaga-nakatsubo K.
Dept. Math. & Life Sci. Grad. Sch. Sci. Hiroshima Univ.
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Akasaka Koji
Laboratory Of Molecular Genetics Department Of Mathematical And Life Sciences Graduate School Of Sci
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Shimada H
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Shimada Hiraku
Laboratory Of Molecular Genetics Department Of Mathematical And Life Sciences Graduate School Of Sci
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Shimada H
Hiroshima Univ. Higashi‐hiroshima Jpn
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Shimada Hiraku
Graduate Department Of Mathematical & Life Sciences Hiroshima Univ.
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Mitsunaga Keiko
Graduate Department Of Mathematical & Life Sciences Hiroshima Univ.
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Akasaka Koji
Graduate Department Of Mathematical & Life Sciences Hiroshima Univ.
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