Effect of Several Redox Dyes on the Respiration of Unfertilized Eggs of Sea Urchin(Developmental Biology)

概要

論文の詳細を見る
The respiration of unfertilized sea urchin eggs became as high in its rate as that of fertilized eggs by adding N, N, N', N'-tetramethyl-p-phenylenediamine (TMPD), phenazine methosulfate (PMS) and dichlorophenol indophenol (DCPIP). The TMPD-stimulated respiration was inhibited by cyanide. The respiration, observed about 10 min after adding PMS, DCPIP or sperm, was inhibited by antimycin A (AMA) and cyanide. During 10 min from adding PMS, DCPIP or sperm, the respiration was insensitive to these inhibitors and became sensitive to them following another addition of TMPD. Cyanide and AMA prolonged the duration of cyanide insensitive respiration. These suggest that electron transport is inhibited in unfertilized eggs in two spans of mitochondrial respiratory chain between flavoprotein and cytochrome b and between cytochrome b and cytochrome c, making the respiratory rate quite low ; the inhibition in the former span is released by adding PMS, DCPIP and sperm, and the inhibition in the latter is gradually eliminated. Following the release of inhibition in the former span, a leakage of electron equivalent from a site between flavoprotein and cytochrome b probably occurs, resulting in cyanide insensitive respiration, which is turned off by the release of inhibition in the latter span. Fertilization also induced another cyanideinsensitive respiration accompanied by peroxide release.
社団法人日本動物学会の論文