Detection of the Centrosomal DNA from the Sperm of Bryophyte Marchantia polymorpha L. and Physcomitrella patens

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概要

• 論文の詳細を見る

• The liverwort Marchantia polymorpha is dioecious and haploid during most of its life cycle. It is known that fertilization in bryophytes, as in animals, involves sperm and eggs. Recently, we isolated novel DNA from the sperm centrosome of the starfish Asterina pectinifera. In the present study, we examined whether the sperm of bryophytes contain this unique DNA. The total DNA in the sperm of M. polymorpha was used as a template for polymerase chain reaction (PCR) analysis performed using a centriole-specific DNA primer. Although this primer was designed to specifically amplify the centrosomal DNA of the starfish, it can amplify the centrosomal DNA of many other animals having a similar DNA sequence. The size of the amplified PCR products was approximately 500 bp, which is similar to that of the PCR products amplified from starfish. We determined the nucleotide sequence similarities between the PCR products amplified from the cultured cells of the moss Physcomitrella patens and from the sperm of the liverwort M. polymorpha. Our results show that DNA similar to that of the starfish centrosomal DNA exists in bryophytes and includes animal centrosomal DNA homologues and candidate centrosomal DNA.

• 神奈川大学の論文
• 2007-05-25

著者

• SANO Takashi

  Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University

• Hino Akiya

  Department of Biological Sciences, Faculty of Science, Kanagawa University

• Hino Akiya

  Kanagawa Univ. Kanagawa Jpn

• Kawai Shinobu

  Research Institute For Integrated Science Kanagawa University:department Of Biological Sciences Facu

• Kawai Shinobu

  Research Institute For Integrated Science Kanagawa University

• Inoue Kazuhito

  Research Institute For Integrated Science Kanagawa University:department Of Biological Sciences Facu

• Hino Akiya

  Research Institute for Integrated Science

• Watai Erika

  Department of Biological Sciences, Faculty of Science, Kanagawa University

• Sano Takashi

  Department Of Biochemistry And Medicine School Of Medicine And Nursing Kitasato University

• Watai Erika

  Department Of Biological Sciences Faculty Of Science Kanagawa University

• Sano Takashi

  Department Of Biological Sciences Faculty Of Science Kanagawa University

• Sano Takashi

  Research Institute for Integrated Science, Kanagawa University

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