Two Types of Na^+/K^+-ATPase Alpha Subunit Gene Transcript in Embryos of the Sea Urchin, Hemicentrotus pulcherrimus
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概要
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We have characterized the nucleotide sequence of Na^+/K^+-ATPase alpha subunit (NKA) cDNA in embryos of the sea urchin, Hemicentrotus pulcherrimus. The primer extension experiments showed that the sea urchin NKA gene generated multiple lengths of transcript. To obtain the 5'-ends of the transcripts, we isolated cDNA clones by the rapid amplification of cDNA ends (RACE). These clones were classified into 2 types on the basis of their 5' leader sequences. The sequences of the clones were identical except their 5' leaders. By Northern blot analysis, 1 of the 2 types of transcripts was always detectable in sea urchin embryos during early development, and another was not detected before the morula stage. Genomic PCR demonstrated that the two 5' leaders were coded by different exons separated by an intron in a single gene. These results show that the transcripts coding 2 isoforms were expressed from a single gene.
- 社団法人日本動物学会の論文
著者
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YAMAZAKI Ken
Department of Pathology, Keio University School of Medicine
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Ihara Toshio
Department Of Biology School Of Education Waseda University
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Yasumasu Ikuo
Department of Biology, School of Education, Waseda University
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Yasumasu I
Department Of Biology School Of Education Waseda University
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Yamazaki K
Univ. Tsukuba Ibaraki Jpn
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Yasumasu Ikuo
Department Of Biology Colege Of General Education University Of Tokyo
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Okamura Chisato
Department of Biology, School of Education, Waseda University
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Okamura Chisato
Department Of Biology School Of Education Waseda University
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Yamazaki Ken
Department Of Electrical And Electronic Engineering College Of Industrial Technology Nihon Universit
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Yamazaki Ken
Department Of Biology School Of Education Waseda University
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