Prediction of Intracellular Amount of 1-Methyladenine Precursor in Ovarian Follicle Cells of the Starfish, Asterina pectinifera(Reproductive Biology)

概要

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Resumption of meiosis in starfish oocytes is induced by 1-methyladenine (1-MeAde) produced by overian follicle cells under the influence of gonad-stimulating substance (GSS). The present study was undertaken to determine whether 1-MeAde production in follicle cells following stimulation by GSS is due to the release of stored 1-MeAde or to de novo synthesis using a precursor of 1-MeAde. Although 1-MeAde produced by follicle cells in Asterina pectinifera was found in the extracellular medium following incubation with GSS, 1-MeAde did not preexist in these cells. Also, the continual presence of GSS in the medium did not maintain 1-MeAde production: as incubation time was prolonged, the level of 1-MeAde production gradually declined and finally stopped. Although 1-MeAde production had already ceased in follicle cells after incubation for 12 hr with GSS, GSS still caused an increase in the intracellular levels of cyclic AMP. It has also been reported that methionine and selenomethionine enhance the GSS-induced 1-MeAde production. In this study it was found that the total amounts of 1-MeAde produced by follicle cells were almost the same regardless of whether methionine or selenomethionine was present. Each follicle cell was capable of producing about 1 fmol 1-MeAde. These results strongly suggested that 1-MeAde is newly synthesized using a precursor stored in follicle cells.
社団法人日本動物学会の論文
1991-02-15