AN IN VIVO STUDY OF DEGRANULATION OF AZUROPHIL GRANULES IN THE NEUTROPHILS DURING PHAGOCYTOSIS OF CATIONIZED FERRITIN IN THE GINGIVAL SULCUS

概要

論文の詳細を見る
Cationized ferritin (CF) was topically applied to the rat gingival sulcus (GS) after hyaluronidase (HY) treatment to investigate morphologically neutrophil responses to foreign substanccs. The tracer could enter into only the junctional epithelium (JE) close to GS and did not penetrate it deeply. Thus, HY did not increase the permeability of the tracer into the JE but induccd marked neutrophil infiltration into the GS. In other words, HY appeared to act as a chemotactic factor for neutrophils rather than a factor that opens the intercellular spaces of the JE. In neutrophils close to the GS which prominently took up CF by phagosomes, the peroxidase-positive azurophil granules were fused with thc CF containingphagosomes, and the granular contents were intracellularly released into the phagosomcs. The part of the phagocytosed CF appeared to be digestcd in the phagosomes, whereas, the extracellular release of the specific granules was not observed. These findings suggest that the foreign substances in the GS are killed and digested by the intracellular release of neutrophil azurophil granules into the phagosomes, namely their intracellular release without impairing the adjacent gingival tissue constituents the first defense line in the GS against invading microbcs.
日本組織細胞化学会の論文