Expression of Alkaline Protease Gene in Bacillus subtilis Mutants That Lack Positive Regulatory Genes degR, degQ, senS, tenA, and proB
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概要
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In addition to the two-component regulatory system, DegS-DegU, five other genes degR, degQ, senS, tenA, and proB positively regulate the production of Bacillus subtilis exoproteases when they are present on a multicopy plasmid. To study the extent of involvement of these genes in exoprotease production in a single copy state and possible relationship among these genes, strains carrying single or multiple disruption mutations in these loci were constructed, and the expression of aprE'-'lacZ was analyzed. From such studies, the following results were obtained with respect to the regulation of aprE expression. i) Disruption mutations were divided into two groups; one (degQ and degR) significantly reduced aprE expression, while the other (senS, tenA, and proB) had little effect on it. ii) SenS was involved in temporal regulation. iii) The combined effects of dome of the disruption mutations tested were not necessarily additive. iv) The extent of the negative effects generated by the mutations depended on the medium used.
- 社団法人日本農芸化学会の論文
- 1997-02-23
著者
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TANAKA Teruo
Department of Oral Anatomy and Cell Biology, Graduate School of Dental Science, Kyushu University
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OGURA Mitsuo
Department of Agricultural Chemistry, The University of Tokyo
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Ogura Mitsuo
Department Of Marine Science School Of Marine Science And Technology
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Tanaka Teruo
Department Of Anatomy Faculty Of Medicine Kyoto University:kansai Medical University
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Tanaka Teruo
Department Of Marine Science School Of Marine Science And Technology
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