DEMONSTRATION OF MEMBRANE-BOUND RECEPTORS BY MEANS OF AUTORADIOGRAPHY
スポンサーリンク
概要
- 論文の詳細を見る
The binding sites of membrane-bound receptors such as insulin, acetyl choline, concanavaline A (Con A) are clearly demonstrated with a radioactive isotope (hot RI) incubation after light fixation. This type of autoradiography (ARG) visualizes the correct binding sites with good morphological characteristics. For steroid hormones such as estrogen including vitamine D_3, the genomic receptor sites cannot be demonstrated with such samples except the essential specific strong binding affinity in the special granules of eosinophilic leucocytes. We demonstrated the binding sites of ^<125>I-insulin in liver, red blood cells, and heart muscle; of ^<125>I-α-bungarotoxin (bungarotoxin) in rat intercostal muscle motor end-plate, cerebellum, and cerebrum; of ^<125>I- or ^3H-Con A receptors on the surface of human red blood cells; and of ^3H-estradiol or ^3H-moxestrol in the special granules of eosinophilic leucocytes in the rat uterus.
- 日本組織細胞化学会の論文
著者
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Suzuki Hidenori
Department Of Cardiovascular Research Tokyo Metropolitan Institute Of Medical Science
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Sugiura Yoshie
Department Of Cell Biology Medical Research Institute Tokyo Medical And Dental University
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Yokofujita Junko
Department Of Cell Biology Medical Research Institute Tokyo Medical And Dental University
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Mizuhira Vinci
Department Of Anatomy & Cell Biology Tokyo Medical & Dental University
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Suzuki Hidenori
Department of Applied Physics, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601
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