CALCIUM TRANSPORT MECHANISM IN CRAYFISH GASTROLITH EPITHELIUM CORRELATED WITH THE MOLTING CYCLE : (I) THE DEMONSTRATION OF CALCIUM-ACCUMULATING SITES
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概要
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The transport of calcium ions in correlation with the molting cycle was studied in the columnar epithelium of the crayfish gastrolith disc tissue by three cytochemical methods. In material fixed by freeze-substitution or potassium oxalate-glutaraldehyde, electron-dense deposits were found only in the hypertrophied mitochondria of the epithelium. In material fixed with potassium antimonate-OsO_4, however, a number of fine deposits were observed throughout all membraneous structures. The emission of calcium K line was demonstrated only in the deposits on the mitochondria in electron probe X-ray microanalysis with the material fixed with acrolein gas, oxalate-glutar-aldehyde, and antimonate-OsO_4. These results may contribute to under-standing the role of mitochondria in calcium transport when a large amount of calcium ions is moved from gastrolith epithelium to the outer molting epithelium or vice versa.
- 日本組織細胞化学会の論文
著者
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Ueno Masaki
Department Of Anatomy & Cell Biology Tokyo Medical & Dental University
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Mizuhira Vinci
Department Of Anatomy & Cell Biology Tokyo Medical & Dental University
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UENO MASAKI
Department of Cell Biology, Medical Research Institute Tokyo Medical and Dental University
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MIZUHIRA VINCI
Department of Cell Biology, Medical Research Institute Tokyo Medical and Dental University
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