Excellent methods for processing crustacean larvae for scanning electron microscopy
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概要
- 論文の詳細を見る
In this study, a novel processing technique that minimizes transformation was developed for observing artemia nauplii by scanning electron microscopy (SEM). The specimens obtained by this method could be observed under very low to high magnification, and minimal morphological transformation was noted in the larval body or in the setae at the tips of the antennae. The technique involves a combination of chemical fixation and microwave irradiation, ultra-high-vacuum freeze-drying, and osmium coating. It could prevent the formation of a charge at the tips of the setae a phenomenon that often occurs during sample processing for SEM. This method may facilitate research on the morphology of crustacean larvae.
著者
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Ueno Masaki
Department Of Anatomy & Cell Biology Tokyo Medical & Dental University
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Ueno Masaki
Department Of Histology Kitasato University School Of Allied Health Sciences
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