剛性の異なる2種類のマウス大腿骨専用骨折作成・プレーティングシステムMouse Fixを用いた骨折治癒過程とFGF-2の発現の比較検討
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概要
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This study aimed to clarify the relationship between the mechanical environment at the fracture site and endogenous fibroblast growth factor-2 (FGF-2). We compared two types of fracture healing with different callus formations and cellular events using MouseFixTM plate fixation systems for murine fracture models. Left femoral fractures were induced in 72 ten-week-old mice and then fixed with a flexible (Group F) or rigid (Group R) Mouse FixTM plate. Mice were sacrificed on days 3, 5, 7, 10, 14, and 21. The callus volumes were measured by 3D micro-CT and tissues were histologically stained with hematoxylin & eosin or safranin-O. Sections from days 3, 5, and 7 were immunostained for FGF-2 and Proliferating Cell Nuclear Antigen (PCNA). The callus in Group F was significantly larger than that in Group R. The rigid plate allowed bone union without a marked external callus or chondrogenesis. The flexible plate formed a large external callus as a result of endochondral ossification. Fibroblastic cells in the granulation tissue on days 5 and 7 in Group F showed marked FGF-2 expression compared with Group R. Fibroblastic cells showed ongoing proliferation in granulation tissue in group F, as indicated by PCNA expression, which explained the relative granulation tissue increase in group F. There were major differences in early phase endogenous FGF-2 expression between these two fracture healing processes, due to different mechanical environments.
- 社団法人 日本実験動物学会の論文
- 2011-01-01
著者
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Steck Roland
Institute Of Health And Biomedical Innovation Queensland University Of Technology
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Urabe Ken
Dep. Of Orthopedic Surgery School Of Medicine Kitasato Univ.
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占部 憲
Department of Orthopedic Surgery, School of Medicine, Kitasato University
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成瀬 康治
Department of Orthopedic Surgery, School of Medicine, Kitasato University
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Ueno Masaki
Department Of Orthopaedic Surgery Kitasato University School Of Medicine
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MINEHARA Hiroaki
Department of Orthopaedic Surgery, Kitasato University School of Medicine
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YAMAMOTO Takeaki
Department of Orthopaedic Surgery, Kitasato University School of Medicine
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GREGORY Laura
Institute of Health and Biomedical Innovation, Queensland University of Technology
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WULLSCHLEGER Martin
Institute of Health and Biomedical Innovation, Queensland University of Technology
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SCHUETZ Michael
Institute of Health and Biomedical Innovation, Queensland University of Technology
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Itoman Moritoshi
Department Of Orthopaedic Surgery Kitasato University School Of Medicine
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Naruse Kouji
Department Of Orthopaedic Surgery Kitasato University School Of Medicine
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Urabe Ken
Department Of Dermatology Graduate School Of Medical Sciences Kyushu University
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Ueno Masaki
Department Of Anatomy & Cell Biology Tokyo Medical & Dental University
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Uchida Kentaroo
Department Of Orthopaedic Surgery Kitasato University School Of Medicine
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Hirano Masahiro
Dep. Of Orthopaedic Surgery Kitasato Univ. School Of Medicine
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Itoman Moritoshi
Kitasato Univ. Sagamihara Jpn
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Yamamoto Takeaki
Department Of Orthopaedic Surgery School Of Medicine Kitasato University
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Steck Roland
Institute Of Health And Biomedical Innovation Institute Of Health And Biomedical Innovation School O
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Schuetz Michael
Institute Of Health And Biomedical Innovation Institute Of Health And Biomedical Innovation School O
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Gregory Laura
Institute Of Health And Biomedical Innovation Queensland University Of Technology
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Wullschleger Martin
Institute Of Health And Biomedical Innovation Queensland University Of Technology
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Uchida Kentaro
Department Of Orthopaedic Surgery School Of Medicine Kitasato University
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Schuetz Michael
Institute Of Health And Biomedical Innovation Queensland University Of Technology
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Ueno Masaki
Department Of Orthopedic Surgery Kitasato University School Of Medicine
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Yamamoto Takeaki
Department Of Orthopaedic Surgery Kitasato University School Of Medicine
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上野 正喜
Department of Orthopaedic Surgery, Kitasato University School of Medicine
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UCHIDA Kentaroo
Department of Biomedical Engineering, Graduate School of Medical Sciences, Kitasato University
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UCHIDA Kentaro
Department of Orthopedic Surgery, Kitasato University School of Medicine
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