マウスプレート固定骨折モデルを用いたGFP骨髄キメラマウスにおける骨髄由来細胞の分布
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概要
- 論文の詳細を見る
To clarify the distribution of bone-marrow-derived cells in fractures treated by plate fixation, fracture models were created using the green fluorescent protein (GFP) chimeric mouse. We observed 2 types of fracture healing processes with different types of callus formation and cellular events by using Mouse Fix™, a device allowing plate fixation on the mouse femur, and differences in the distribution of bone-marrow-derived cells between the 2 types. The GFP chimeric mice were created by bone marrow transplantation. Fractures were created on the left femurs of mice and stabilized with either rigid (Group R) or flexible (Group F) plates to prepare undecalcified fresh-frozen sections. In Group F, a large external callus and a large intramedullary callus were formed mostly by endochondral ossification. The cells that made up the intramedullary callus and callus in the fracture gap were GFP positive, but most cells of the external callus were not. In Group R, bone union was achieved mostly without external callus formation, bone apposition occurred directly in the gap, and a small intramedullary callus was formed. As observed in Group F, this group had GFP-positive cells in the callus within the fracture gap and in the intramedullary calluses. The results of this study provided direct evidence of the distribution of bone-marrow-derived cells in the callus of fractures treated by plate fixation under different stability conditions.
著者
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Takaso Masashi
Kitasato Univ. School Of Medicine
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上野 正喜
北里大学医学部生化学
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Itoman Moritoshi
Department Of Orthopaedic Surgery Kitasato University School Of Medicine
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Takahira Naonobu
Kitasato Univ. Sagamihara Jpn
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Steck Roland
Institute Of Health And Biomedical Innovation Institute Of Health And Biomedical Innovation School O
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Schuetz Michael
Institute Of Health And Biomedical Innovation Institute Of Health And Biomedical Innovation School O
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UENO Masaki
Kitasato University School of Medicine, Department of Orthopaedic Surgery
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UCHIDA Kentaro
Kitasato University School of Medicine, Department of Orthopaedic Surgery
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MINEHARA Hiroaki
Kitasato University School of Medicine, Department of Orthopaedic Surgery
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SUTO Kaori
Kitasato University School of Medicine, Department of Orthopaedic Surgery
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