高週齢マウス大腿骨骨折モデルにおける低出力超音波パルスの内軟骨性骨化の促進効果
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概要
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The aim of this study is to clarify the effect of low intensity pulsed ultrasound (LIPUS) on shortening of the fracture healing period and endochondral ossification during the fracture healing process. We first established a model of aging-related delayed union fractures consisting of aged mouse (C57BL/6J; 40 weeks old) with closed femur fractures. We compared the healing process of 40-week-old mice to the healing process of 8-week-old (young) mice using radiological and histological analysis. In aged mice, some cartilage formation was observed 10 days after the fracture; however, endochondral ossification and hard callus bridging were observed 21 and 28 days after the fracture, respectively, whereas cartilage remained in the callus on day 28, suggesting delayed endochondral ossification following bone remodeling. Meanwhile, in aged mice with LIPUS treatment, cartilage formation was similar to that in aged mice without LIPUS; however, hard callus bridging and bone remodeling were observed 21 and 28 days after fracture, respectively, suggesting that LIPUS shortened the healing period due to promotion of endochondral ossification. Immunohistochemical analysis showed marked expression of vascular endotherial growth factor and neovascularization in the fibrous tissue comprising the periosteum that surrounded the whole callus. A cell migration test involving primary cultured human endothelial cells also showed promotion of cell migration by LIPUS. These results suggested that endothelial cell migration and neovascularization, which were observed around fracture sites, played a part in the mechanism of promotion of endochondral ossification by LIPUS.
- 2011-07-01
著者
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高相 晶士
北里大学東病院整形外科
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Takaso Masashi
Department Of Orthopaedic Surgery School Of Medicine Chiba University
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Urabe Ken
Dep. Of Orthopedic Surgery School Of Medicine Kitasato Univ.
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内田 健太郎
Department of Biomedical Engineering, Graduate School of Medical Sciences, Kitasato University
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成瀬 康治
Department of Orthopedic Surgery, School of Medicine, Kitasato University
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Naruse Kouji
Department Of Orthopaedic Surgery Kitasato University School Of Medicine
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Urabe Ken
Department Of Dermatology Graduate School Of Medical Sciences Kyushu University
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Uchida Kentaroo
Department Of Orthopaedic Surgery Kitasato University School Of Medicine
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Takaso Masashi
Department Of Orthopaedic Surgery Kitasato University School Of Medicine
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Itoman Moritoshi
Kitasato Univ. Sagamihara Jpn
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ITOMAN MORITOSHI
Kyushu Rosai Hospital
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KATANO Motoaki
Departments of Orthopedic Surgery, Kitasato University School of Medicine
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Mikuni Takagaki
Department Of Functional Biology Kanagawa Dental College
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Takaso Masashi
Departments Of Orthopedic Surgery Kitasato University School Of Medicine
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Mikuni Takagaki
Department Of Maxillofacial Diagnostic Science And Functional Biology Kanagawa Dental College
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Uchida Kentaro
Department Of Orthopaedic Surgery School Of Medicine Kitasato University
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Takaso Masashi
Department Of Orthopedic Surgery Kitasato University School Of Medicine
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片野 素昭
Departments of Orthopedic Surgery, Kitasato University School of Medicine
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Katano Motoaki
Departments Of Orthopedic Surgery Kitasato University School Of Medicine
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UCHIDA Kentaro
Department of Orthopedic Surgery, Kitasato University School of Medicine
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