STRONG AFFINITY OF ESTRADIOL AND ITS LIGANDS FOR EOSINOPHILIC LEUCOCYTES IN THE RAT AND MOUSE UTERI DURING THE ESTROUS CYCLE

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概要

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• The affinity of estradiol (E_2) for eosinophils in the rat and mouse uteri during the estrous cycle was studied by light and electron microscope autoradiography (ARG). In in vivo ARG, in which the prefixed uteri were incubated at each stage of estrous cycle with ^3H-E_2 or its ligand ^3H-moxestrol, the developed silver grains were localized on the specific granules of eosinophils. At the late estrus phase, eosinophils were frequently phagocytized by macrophage-like interstitial cells, whose phagosome included eosinophilic granules in various stage of degeneration covered with silver grains, and also showed positive reaction for acid phosphatase activity. Grain accumulation was inhibited when the tissue was incubated in ^3H-E_2 with a 100-fold excess of nonradioactive E_2. When fresh frozen thin sections were incubated in ^3H-E_2, we succeeded in demonstrating the binding of E_2 to the eosinophils in spleen, small intestine, blood and bone marrow. However, in in vivo ARG using the fresh thaw-mount technique, only the uterine eosinophils showed grain accumulation, and eosinophils of other tissues did not bind ^3H-E_2. Local eosinophilia was observed in the uterus of ovariectomized rats at one hr after E_2 injection into the uterine wall. These results suggest that all eosinophils may have a strong affinity for E_2, and that they are directly induced into the uterus by estrogen. Specific granules of eosinophils, which contain strong peroxidase enzymatic activity, seem to destroy the excess amount of E_2 in the uterus at estrus, and then the eosinophils are phagocytized by the macrophage-like interstitial cells.

• 日本組織細胞化学会の論文

著者

• Sugiura Y

  Department Of Endocrinology Medical Research Institute Tokyo Medical And Dental Universify

• Sugiura Yoshie

  Department Of Cell Biology Medical Research Institute Tokyo Medical And Dental University

• Mizuhira Vinci

  Department Of Anatomy & Cell Biology Tokyo Medical & Dental University

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