Identification of RNA as a substance responsible for the survival of chick spinal motoneurons in vitro
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概要
- 論文の詳細を見る
In our preceding paper, we presented the purification of a chick embryos muscle-derived substance that prevented spinal neurons from undergoing cell death in vitro. In this study, characterization of this substance by 1H-NMR spectroscopic analysis and enzymatic inactivation experiments revealed that the substance responsible for the survival of cultured spinal neurons was RNA. For further characterization a modified purification procedure was devised on the basis of the substance being RNA. The finally purified substance showed λmax at 258nm and prevented enriched populations of spinal motoneurons from undergoing cell death in vitro, in the same way as the crude muscle extract. These results suggested a novel role of RNA as an intercellular signaling molecule.
- 日本学士院の論文
著者
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SUZUKI Hidenori
Department of Dermatology, Graduate School of Biomedical Sciences, Hiroshima University
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Suzuki Akinori
Graduate School of Agriculture, Hokkaido University
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Suzuki Hidenori
Department Of Cardiovascular Research Tokyo Metropolitan Institute Of Medical Science
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Ui-tei Kumiko
Depatrment Of Pharmacology Nippon Medical School
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TAKAMIYA Masanari
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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UI-TEI Kumiko
Department of Pharmacology, Nippon Medical School
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NAGANO Masatoshi
Department of Pharmacology, Nippon Medical School
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MIYATA Yuhei
Department of Pharmacology, Nippon Medical School
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KATAOKA Hiroshi
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Miyata Yuhei
Department Of Pharmacology Nippon Medical School
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Nagano Masatoshi
Department Of Pharmacology Nippon Medical School
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Suzuki Hidenori
Department Of Cardiovascular Research The Tokyo Metropolitan Institute Of Medical Science
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Suzuki Hidenori
Department of Applied Physics, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601
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SUZUKI Akinori
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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