グアガム分解細菌の同定とグアガム分解酵素生産条件

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A guar gum-degrading bacterium isolated from soil and previously identified as Pseudomonas sp. No. 37 was re-identified as Bacillus circulans. The bacterium was classified into strains of two types, without (strain K-1) with (strain K-4)spore forming ability. The secretion and production conditions of the guar gum-degrading enzyme were investigated with strain K-1. The bacterium inductively secreted the GG-degrading enzyme in a culture liquid that included GG, but the enzyme was not secreted when the culture liquid did not contain GG. Intra-and extra-cellular enzyme activities were compared by measuring the units of enzymatic activity. In a 24-h culture at 30℃ the intra-and extra-cellular enzyme activities were 0.043U/ml and 1.7U/ml, respectively. The enzyme was not secreted in the culture medium containing a mixture of GG and sugar as fructose or glucose. The bacterium secreted an enzyme that mutually degraded GG, spino gum (SG) and locust bean gum (LG) when the culture medium included a galactmannam as SG and LG. The enzymatic secretion in a shaking culture at a speed of 80-150 min^<-1> was about double that of the standing culture, but the amount secreted per unit bacterial mass was almost the same. The enzymatic secretion was maximum when the culture was carried out at a pH value between 7.0 and 7.4 and at 30℃. In a synthetic medium with guar gum as the sole source of carbon, the number of bacteria and the amount of enzymatic secretion in the culture liquid were proportional to the guar gum concentration.
社団法人日本生物工学会の論文
1994-07-25

グアガム分解細菌の同定とグアガム分解酵素生産条件

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