2,2'-ビピリジルを用いるガリウム(III)の蛍光定量

概要

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In a fluorometric determination a lower fluorescence intensity of excess reagent allows for a higher sensitivity. The fluorescence intensity of 2,2'-bipyridyl (bipy) is lower than that of 1,10-phenanthroline, which is the typical chelate reagent of N, N coordination. Therefore, the fluorometric properties of metal complexes formed with bipy were examined in an aqueous solution in order to determine metals by fluorometry with bipy. As a result, it was found that gallium reacted with bipy to form a fluorescence complex, which yielded fluorescence emission with an excitation wavelength of 305 nm, an emission wavelength of 325 nm, a fluorescence quantum yield of 0.042 and a molar absorptivity of 12200 l/cm mol at 305 nm. The detection limit of 0.8 ppb was accomplished under the most suitable conditions (pH of 5.0,temperature of 25℃, and bipy concentration of 3.8×10^<-5>M). Though the fluorescence quantum yield of the complex was quite small. the lower detection limit mentioned above was obtained due to the low fluorescence intensity of bipy.
社団法人日本分析化学会の論文
1997-05-05