無電極型水晶発振子を用いる一滴法によるシュウ酸イオンの定量

概要

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The single drop method using an electrcdeless piezoelectric quartz crystal (PQC) was able to be applied to the determination of oxalate, in the form of calcium oxalate, in spinach extract. Setting an electrodeless quartz plate, which was treated with paraffin, on the copper plate electrode in a cell, a drop of 5μl of calcium chloride solution containing potassium chloride and acetate buffer was applied. A platinum wire of a counter electrode was inserted into the drop at 1.0mm distance between the plate and the top of the wire electrode, and the system was left until the oscillation frequency of the electrodeless PQC became constant. The oxalate sample solution of 5μl containing potassium chloride and acetate buffer was then added to the drop. By reading the frequency change between just after the application of the sample solution and at 3min later, the concentration of oxalate was estimated. Oxalate in spinach leaves was extracted with hot water by boiling for 20min. After passing through a filter paper, the extract was added to acetic acid and incubated over night. Then the extract was filtrated using a membrane filter, and to it was added potassium chloride and sodium acetate to adjust the pH to 4.6. This solution was used as the sample. The concentration of oxalate in the spinach leaves estimated by this method was in agreement with that obtained by ion chromatography.
社団法人日本分析化学会の論文
1994-09-05