Saccharomyces cerevisiae菌浮遊液によるデスチオビオチンの生合成 : (VII)ブドウ糖のビオチンの生成促進効果

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The stimulative action of glucose in the formation of biotin from desthiobiotin by washed cells of Saccharomyces cerevisiae was investigated. The biotin-deficient cells took up a greater amount of desthiobiotin than the biotin-sufficient cells, and then the intracellular levels of biotin became approximately constant. The uptake of desthiobiotin by washed cells increased markedly by the addition of glucose, and the effect was inhibited by the addition of iodoacetic acid or dinitrophenol. With the increase of desthiobiotin concentration in a incubation system without glucose, the uptake increased proportionally, but the biotin formation did not increase. The uptake of desthiobiotin by the washed cells is appeared to occur by two mechanisms which are glucose-independent and -dependent. The rate of biotin formation from desthiobiotin in the optimal temperature raised approximately 2.5 fold by the addition of glucose. It was suggested that the formation of biotin from desthiobiotin required further energy source.
1967-04-25