ペニシリン生産菌株Wis-Q-176表面培養に關する研究(續) : 實驗 IX〜V

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We studied on the surface cultuse of P. chrysogenum Q-176 with Stone and Farrell synthetic medium, and obtained the following results. 1. In order to utilize the stimulating substances of the mycelial mat obtained from the surface culture, we analyzed their general constituents, and observed their changes during the autolysis. When two solutions were used for penicillin fermentation one of which was made by the autolysis of the mycelial mat, and the other was made by digestion with diastese, the penicillin production of the latter was better than that of the former. 2. We wxamined the chemical changes during the surface culture with P. chrysogenum Q-176 in Stone and Farrell medium. The results are as follows : - The lactose utilization was rapid and almost complete by 12 day culthre. Total nitrogen decreased gradually, and became minimum on the 9th day, and then seemingly increased. Perhaps, these phenomena are owing to the autolysis of the mycelial mat, for the weight of mycelium increased gradually for 9 days, and then began to decrease. The change of acetic acid was remarkable. Namely, about 95% of acetic acid was almost consumed in 6 days. THerefore, necessarily pH began to rise, and then reached to 7.9 from initial pH 5.7 for 3 days, and then there was no change. As we discussed in exp. 3,the buffer action is increased by adding of acetic acid, but as we descrived above, owing to its rapid consumption in the fermentation pH becomes alkaline. Therefore, we think that acetic acid is utilized as the nutrient rather than buffer action by this mould.
社団法人日本生物工学会の論文
1949-04-15

ペニシリン生産菌株Wis-Q-176表面培養に關する研究(續) : 實驗 IX〜V

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