高分子抗生物質SN-07のクロマトグラフィー精製

概要

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A macromolecular antibiotic, SN-07,was purified from the cultural supernatant of Actinomadura roseoviolace var. miuranensis nov. var. by the successive staps of salting-out, ethanol precipitation, ion exchange chromatography (IEC), hydrophobic chromatography (HIC), hydroxyapatite chromatography, and gel filtration chromatography.In conventional IEC and HIC, SN-07 was purified by linear gradient elution (LGE) with increasing NaCl concentration for IEC on DEAE-Sephadex A-25 and decreasing ammonium sulfate concentration for HIC on Phenyl-Sepharose CL-4B.For effective purification on a large scale, however, it is necessary to use a simple elution method such as stepwise elution (SE) and to operate at a high linear velocity with suitable selection of adsorbent gel. In this work Sepabeads FP-DA 13 and FP-PH 12,which have higher mechanical strength than Sephadex and Sepharose beads, were used for IEC and HIC, respectively, and SN-07 was purified by SE in these chromatographic steps. In SE the yield and purity of SN-07 were almost the same as or higher than those in LGE, and the total operation time was reduced to one third. Moreover the concentrations of SN-07 fractions by SE from IEC and HIC were 3.4 and 1.5 times those of LGE, respectively.
社団法人日本生物工学会の論文
1987-11-25