微生物におけるアミノ酸-アルデヒドアミノ基転移反応

概要

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L-Alanine-aldehyde transamination by cell-free extracts of microorganisms was investigated. Aspergillus oryzae, Saccharomyces cerevisiae (Kyokai no. 6), Lactobacilli and nitrate-reducing bacteria were used. These organisms are used in sake brewing. All the tested strains showed transaminating activity. Particularly, three strains of nitrate-reducing bacteria, Aerobacter aerogenes C, Achromobacter guttatus D and Bacillus subtilis S7,showed high activities and broad substrate specificities for aldehydes. The addition of aldehyde to the medium didn't increase the activity.Properties of L-alanine-n-butyraldehyde transamination by the cell-free extract of B. subtilis S7 were studied. The pH optimum was found to be about 8.0-8.5. The optimal temperature was found to be 27.5-30.0℃. The Km values were 6.0×10^<-2>M for L-ananine and 5.5×10^<-2>M for n-butyraldehyde. The addition of pyridoxal phosphate increased the activity. Several keto acids and carbonyl reagents showed high inhibition.
社団法人日本生物工学会の論文
1982-07-25