イネ白葉枯病菌培養液中に見出されたイネ苗幼根生育抑制物質,フェニール酢酸について

概要

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A growth-depressing substance to young roots of rice seed11ngs was assumed to be existed in the culture medium which was inoculated with Xanthomonas oryzae. In order to isolate the active principle in pure crystal state and to identify chemica11y, the fo11owing procedures were adopted : The Wakimoto's potato semi-synthetic culture medium was prepared, inoculated with the causal bacterium and kept at 28℃ in shaking conditions. Seven days after, the culture solution was extracted with ethylether and then re-fractionated to acidic part. After steam-dist111ation, the residue was submitted to columnchromatography over si11ca gel, to elute the bio1ogica11y active zone by chro1oform and to fract1onate by fraction collecter. Fina11y, about 50 mg of the crystal was obtained in pure state from 10l of the inoculated culture medium by the above-mentioned procedures. The melting point lies at 76.5℃ and was not depressed by admixture with authentic phenylacetic acid. The substance was identica1 in infrared spectrum with authentic phenylacetic acid. Judging from these characters, the authors concluded that the growth-depressing substance to young roots of rice seed1ings which was produced by X. oryzae in culture was identical with phenylacetic acid.
日本植物病理学会の論文
1968-01-31