アミノ酸含有培地に植物病原菌を培養した場合に生産される未知ニンヒドリン陽性物質(2)

概要

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In the previous paper it was reported that a ninhydrin-positive substance (NPS) is produced in a modified Czapek's medium, in which NaNO 3 was replaced by an amino acid, inoculated with several plant pathogenic fungi. NPS is produced non-bio1ogically in the modified Czapek-tryptophan medium when the medium was adjusted at pH 2.8 or 4.8, whereas it was not produced non-biologica11y at higher hydrogen-ion concentrations, 7.0, 9.0 and 11.O. NPS was produced in the same medium even at high hydrogen-ion concentrations, 6.8, 7.2, 8.2 and 9.O, when inoculated with plant pathogenic fungi such as Alternaria kikuchiana, CladosPorium herbarum and Colletotrichum dematium. In order to check the non-biological production of NPS at the acidic micro-environmentsdis tributed 1oca11y on the myce1ial surface in 1iquid medium, silica gel particles were added, in place of fungal myce11um, to the medium and the production of NPS was tested, however, no NPS was detected. These results indicate that NPS is produced by the fungi through some metabo1ic process. NPS is produced in the medium within two days, reaching its maximum about 6th day after inoculation, at a wide pH range, from 6.8 to 9.O. In order to identify NPS, its behavior on thin layer chromatography was compared with those of artificia11y synthesized N-glycosides. The result indicated that NPS contains fructose as sugar moiety.
日本植物病理学会の論文
1968-09-30