Optimization of Heterotrophic Culture Conditions for n-Alkane Utilization and Phylogenetic Position Based on the 18S rDNA Sequence of a Thermotolerant Prototheca zopfii Strain

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This study reports on the optimization of the culture conditions for a thermotolerant eukaryotic algal strain, Prototheca zopfii RND16, which can effectively degrade and assimilate n-alkanes at elevated temperatures. RND16 was able to grow on 1% (v/v) n-alkanes (C_14-C_17) at temperatures up to 38℃. This ability differs from a previous finding that P. zopfii did not grow on hydrocarbons under temperature conditions above 25℃. Increasing the temperature from 25℃ to 30-35℃ resulted in an increase in the rate of n-alkane consumption during growth of RND16 in quiescent culture. In shaking culture at 35℃, RND16 removed a 1% n-alkane mixture almost completely at the basal medium salinity within 8 d. However, an appreciable decrease in the extent of hydrocarbon utilization was observed with increasing salinity and substrate concentration in the medium. The slow consumption of the n-alkanes in the early stage of degradation at 25℃ was improved by supplementing 0.1% glucose. A comparative study on the nuclear small subunit rDNA (18S rDNA) sequences of three strains within the genus Prototheca revealed that both P. zopfii ATCC 30253, which utilize n-alkanes at room temperature, and P. moriformis ATCC 50081, which does not assimilate n-alkanes, are closely related to RND16.
社団法人日本生物工学会の論文
2002-08-25