Fluorescent Antibody Study of Chitobiase in Uninfected and Nuclear-Polyhedrosis-Virus Infected Larvae of the Silkworm, Bombyx mori (Lepidoptera : Bombycidae)

概要

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Fluorescent antibody studies were conducted on the distribution of chitobiase in silk-worm larvae, Bombyx mori, non-infected or infected with a nuclear polyhedrosis virus (NPV). Chitobiase, in non-infected control larvae, appeared first in the small tracheae and then in the large tracheae, anterior silkgland, foregut, hindgut, and integument in the process of larval-larval transformation. The enzyme rapidly disappeared when the larvae underwent ecdysis. A specific fluorescence of chitobiase was observed only on the chitinolytic tissues but not within their cells. In NPV-infected larvae, the distribution of chitobiase was similar to that in the control larvae, but the specific fluorescence was continuously observed when the larval ecdysis was blocked by the multiplication of NPV.
日本応用動物昆虫学会の論文
1985-05-25