Characterization of Non-endcapped Polymeric ODS Column for the Separation of Triacylglycerol Positional Isomers
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概要
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The characteristics of a non-endcapped polymeric ODS column for the resolution of triacylglycerol positional isomers (TAG-PI) were examined using a recycle HPLC-atmospheric pressure chemical ionization/mass spectrometry system. A pair of TAG-PI containing saturated fatty acids at least 12 carbons was separated. Except for TAG-PI containing elaidic acid, pairs of TAG-PI containing three unsaturated fatty acids were not separated, even by recycle runs. These results indicate that the resolution of TAG-PI on a non-endcapped polymeric ODS stationary phase is realized by the recognition of the linear structure of the fatty acid and the binding position of the saturated fatty acid in TAG-PI. Chain length was also an important factor for resolution. This method may be a useful and simple for measuring the abundance ratio of TAG-PI containing saturated fatty acids in natural oils.
著者
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Nagai Toshiharu
Tsukishima Foods Industry Co., Ltd.
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Ichioka Kenji
Tsukishima Foods Industry Co., Ltd.
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MATSUMOTO YUMIKO
Department of Anesthesiology Hamamatsu Rosai Hospital
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Noguchi Noriko
Department Of System Biology And Medicine Research Center For Advanced Science And Technology The Un
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WADA Shun
Department of Food Science and Technology, Tokyo University of Fisheries
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GOTOH Naohiro
Department of Food Science and Technology, Tokyo University of Fisheries
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Wada Shun
Department Of Applied Biological Chemistry Faculty Of Agriculture Shizuoka University
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Gotoh Naohiro
Department Of Food Science And Technology Tokyo University Of Fisheries
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KURODA Ikuma
GL Sciences Inc.
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Yuji Hiromi
Department of Food Science and Technology, Tokyo University of Marine Science and Technology
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Mizobe Hoyo
Tsukishima Foods Industry Co. Ltd.
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Gotoh Naohiro
Department of Food Science and Technology, Tokyo University of Marine Science and Technology
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Ichioka Kenji
Tsukishima Foods Industry Co. Ltd.
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Noguchi Noriko
Department of Medical Life Systems, Doshisha University
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Nagai Toshiharu
Tsukishima Foods Industry Co. Ltd.
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