Mathematical Approach to Thermolysin-Catalyzed Synthesis of Aspartame Precursor
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概要
- 論文の詳細を見る
Aspartame precursor (N-(benzyloxycarbonyl)-L-aspartyl-L-phenylalanine methyl ester), an artificial sweetener, was synthesized enzymatically from N-(benzyloxycarbonyl)-L-aspartic acid (Z-L-Asp) and L-phenylalanine methyl ester (L-PM) as substrates with thermolysin as an enzyme in an aqueous monophase system. A reversible Theorell-Chance mechanism is proposed to explain the synthesis, and its kinetic parameters were determined at pH 6.5 and 40°C. The rate equation of the enzymatic reaction, which has never been solved with respect to the concentration of the product, was solved with effective approximations. The equation obtained was more practical than the general integrated form of rate equations, and the calculated results could satisfactorily explain the experimental results. The synthesis of aspartame precursor can thus be said to be based on the reversible Theorell-Chance mechanism. It is considered that this effective method of approximation can be applied to other enzymatic reactions.
- 社団法人日本生物工学会の論文
- 1996-09-25
著者
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MURAKAMI Yoshihiko
Department of Pathobiology, College of Bioresource Sciences, Nihon University
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HIRATA Akira
Department of Medical Biochemistry, Graduate School of Medical Science, Kyushu University
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Murakami Y
School Of Materials Science Japan Advanced Institute Of Science And Technology
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Murakami Yoshihiko
Department Of Chemical Engineering Waseda University
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HIRATA MAKOTO
Department of Applied Chemistry, Oita University
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Hirata Akira
Department Of Chemical Engineering Waseda University
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Hirata Makoto
Department Of Applied Chemistry Oita University
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Hirata Makoto
Department of Agricultural Chemistry, Faculty of Agriculture, Kyushu University
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