Characterization of Denitrifying Polyphosphate-Accumulating Organisms in Activated Sludge Based on Nitrite Reductase Gene(Environmental Biotechnology)
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概要
- 論文の詳細を見る
Nitrite reductase gene (nirS) fragments in the activated sludge obtained from a sequencing batch reactor (SBR) under anaerobic-aerobic condition were cloned and classified by restriction fragment length polymorphism (RFLP) analysis, and representative fragments were sequenced. One of the nirS clones was approximately 70% of all nirS clones in anaerobic/aerobic (existing oxygen and nitrate) cycle operation in which a large amount of anoxic phosphate uptake was observed. Although the activated sludge samples analyzed might contain bacteria that did not accumulate polyphosphate, it was likely that this nirS fragment sequence was that from denitrifying polyphosphate-accumulating organisms (DNPAOs) which can utilize both oxygen and nitrate as electron acceptors. The sequence was similar to the nirS sequences of Thauera mechernichensis (83% similarity) and Azoarcus tolulyticus (83% similarity) both of which belong to the Rhodocyclus group.
- 公益社団法人日本生物工学会の論文
- 2005-04-25
著者
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Tsuneda Satoshi
Department of Life Science and Medical Bioscience, Waseda University
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HIRATA Akira
Department of Medical Biochemistry, Graduate School of Medical Science, Kyushu University
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常田 聡
早稲田大学理工学部生命医科学科
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OHNO Takashi
Department of Psychiatry, Tohoku University Graduate School of Medicine
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Tsuneda Satoshi
Department Of Chemical Engineering Waseda University
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Ohno T
Department Of Quantum Materials Science University Of Tokushima
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MIYAUCHI RYUKI
Department of Chemical Engineering, Waseda University
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Hirata Akira
Department Of Chemical Engineering Waseda University
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Ohno Takashi
Department Of Agrobioscience Graduate School Of Agriculture Kobe University
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Miyauchi Ryuki
Department Of Chemical Engineering Waseda University
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