Microbial Community Analysis in the Denitrification Process of Saline-Wastewater by Denaturing Gradient Gel Electrophoresis of PCR-Amplified 16S rDNA and the Cultivation Method
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概要
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The metallurgic wastewater generated from the processes of recovering precious metals from industrial wastes contains high concentrations of nitrogen compounds and salts. Biological nitrogen removal from this wastewater was attempted using a circulating bioreactor system equipped with an anaerobic packed bed or an anaerobic fluidized bed. The denitrification capability of the system with the anaerobic packed bed was more stable than that of the system with the anaerobic fluidized bed. The NOx removal rate of the anaerobic packed bed was as high as 97%. Microbial community analysis by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA (rDNA) fragments and the cultivation method revealed that the community diversity varied in accordance with wastewater composition such as the level of salinity and so on. Phylogenetic analysis suggested that the taxonomic affiliation of the dominant species in the anaerobic reactors was to the γ-Proteobacteria including Halomonadaceae species. The PCR-DGGE method as a non-cultivation method was found to be a powerful tool for analysis of the microbial community, because the cultivation method could detect only a fraction of the microbial species present in these systems. The genetic diversity of the isolated bacteria belonging to the γ-Proteobacteria which reduced both nitrate and nitrite in the anaerobic packed bed was higher than that of the bacteria in the anaerobic fludized bed. This suggested that a genetically diverse microbial community stabilized the denitrifying performance in the anaerobic packed bed.
- 社団法人日本生物工学会の論文
- 2001-10-25
著者
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Tsuneda Satoshi
Department of Life Science and Medical Bioscience, Waseda University
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HIRATA Akira
Department of Medical Biochemistry, Graduate School of Medical Science, Kyushu University
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常田 聡
早稲田大学理工学部生命医科学科
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MIYANO Tomoko
Department of Physics. Aoyama-Gakuin University
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Miyano Tomoko
Department Of Chemical Engineering Waseda University
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Inamori Yuhei
National Institute for Environmental Studies
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Inamori Y
National Institute For Environmental Studies
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Inamori Yuhei
Regional Environment Division National Institute For Environmental Studies
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Tsuneda Satoshi
Department Of Chemical Engineering Waseda University
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Noda Naohiro
Institute For Biological Resources And Functions National Institute Of Advanced Industrial Science A
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YOSHIE SACHIKO
Department of Chemical Engineering, Waseda University
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NODA NAOHIRO
Department of Chemical Engineering, Waseda University
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Yoshie Sachiko
Department Of Life Science And Medical Bioscience Waseda University
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INAMORI Yuhei
Fukushima Univ.
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Hirata Akira
Department Of Chemical Engineering Waseda University
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Inamori Yuhei
Facility Of Symbiotic System Science Fukushima University
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