Purification and Characterization of o-Hydroxyphenylacetate 5-Hydroxylase, m-Hydroxyphenylacetate 6-Hydroxylase and p-Hydroxyphenylacetate 1-Hydroxylase from Rhodococcus erythropolis

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概要

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• The gram-positive Rhodococcus erythropolis strain S1 was found to utilize o-, m-, and p-hydroxyphenylacetic acids as sole carbon sources. Each isomer of monohydroxyphenylacetate was degraded via the homogentisate pathway, which contained a reduced glutathione independent-isomerase. Three monohydroxyphenylacetate monooxygenases, o-hydroxyphenylacetate 5-hydroxylase, m-hydroxyphenylacetate 6-hydroxylase, and p-hydroxyphenylacetate 1-hydroxylase, were purified to homogeneity from strain S1. Each enzyme was a 45-kDa monomeric NADH-dependent monooxygenase containing FAD, and all three appeared to belong to the p-hydroxybenzoate hydroxylase-class as regards the flavin-containing aromatic compound monooxygenase family. However, the three enzymes differed greatly in terms of substrate specifictiy.

• 社団法人日本生物工学会の論文
• 1996-02-25

著者

• NAKAJIMA Kenji

  Japan Atomic Energy Agency

• NAKAMURA Yoshihiro

  National Space Development Agency of Japan

• Suemori A

  National Inst. Biosci. And Human‐technol. (nibh) Ibaraki Jpn

• Suemori Akio

  National Institute Of Bioscience And Human-technology (nibh)

• KURANE Ryuichiro

  National Institute of Bioscience and Human-Technology (NIBH)

• NAKAJIMA KENJI

  National Institute of Bioscience and Human-Technology(NIBH)

• SUEMORI AKIKO

  National Institute of Bioscience and Human-Technology (NIBH)

• Nakajima K

  Japan Atomic Energy Agency

• Suemori A

  National Institute Of Bioscience And Human-technology (nibh)

• Nakajima K

  National Inst. Biosci. And Human‐technol. Ibaraki Jpn

• Nakamura Y

  Ajinomoto Co. Inc. Jpn

• Kurane R

  National Inst. Advanced Industrial Sci. And Technol. Tsukuba Jpn

• Nakajima Kenji

  National Institute Of Bioscience And Human-technology

• Kurane Ryuichiro

  National Institute Of Biosciecne And Human Technology Agency Of Industrial Science And Technology Ministry Of International Trade & Industry

• Nakamura Yoshihiro

  National Institute of Bioscience and Human-Technology (NIBH)

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