Simple and Cost-Effective Procedure for Purification of Biopolymer from Highly Viscous Alcaligenes latus Culture Broth
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概要
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A fractional precipitation procedure was modified and applied to the purification of a biopolymer (bioabsorbent) from a highy viscous Alcaligenes latus B-16 culture broth. Cells were removed by filtration using a 2-propanol concentration of about 33%. The biopolymer (bioabsorbent) was recovered using 66% 2-propanol by filtration. The nitrogen and ash content at each step of the fractional precipitation procedure were determined to confirm the purify of the biopolymer (bioabsorbent). Using this procedure, cells and salts were easily and rapidly removed from the biopolymer (bioabsorbent) in a small volume.
- 公益社団法人日本生物工学会の論文
- 1996-01-25
著者
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Mita Hajime
Department of Chemistry, University of Tsukuba
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Mita H
Department Of Chemistry University Of Tsukuba
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KURANE Ryuichiro
National Institute of Bioscience and Human-Technology (NIBH)
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Mita Hajime
Tsukuba Research Laboratory Japan Synthetic Rubber Co. Ltd.:(present Address)department Of Chemistry
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Kurane Ryuichiro
National Institute Of Biosciecne And Human Technology Agency Of Industrial Science And Technology Ministry Of International Trade & Industry
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Kurane Ryuichiro
National Institute Of Bioscience And Human-technology Agency Of Industrial Science And Technology
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