Production of a Bioflocculant by Mixed Culture
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概要
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Colony groups that form large amounts of slime externally were obtained from activated sludge among phthalate-assimilating microbes. That slime, which R-3 mixed microbes produced externally, had a high level of flocculation activity. R-3 mixed strains, one of such colony groups, efficiently produced a bioflocculant (APR-3) in liquid cultures of production medium, especially that containing starch and glucose (1 : 1) as carbon sources. Identitication of this group of bioflocculant-producing microbes (R-3 mixed strains) showed that it was comprised of four strains belonging to the genera Oerskovia, Acinetobacter, Agrobacterium, and Enterobacter. The bioflocculant (APR-3) was purified electrophoretically to homogeneity by ethanol and CPC precipitation. Its molecular mass is at least 2×10^6 Da. APR-3 is an acidic polysaccharide made of glucose, galactose, succinic acid, and pyruvic acid (molar ratio : 5.6 : 1 : 0.6 : 2.5).
- 社団法人日本農芸化学会の論文
- 1994-09-23
著者
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KURANE Ryuichiro
National Institute of Bioscience and Human-Technology (NIBH)
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MATSUYAMA Hideaki
Department of Engineering Science,Faculty of Engineering,Hokkaido University
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Matsuyama H
Kankyo Engineering Corporation
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Matsuyama Hideaki
Kankyo Engineering Corporation
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Kurane Ryuichiro
National Institute Of Biosciecne And Human Technology Agency Of Industrial Science And Technology Ministry Of International Trade & Industry
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