Culture Conditions for Production and Purification of Bioabsorbent from Alcaligenes latus B-16 : Biopolymers from A. latus (III).
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概要
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Glucose and sucrose were favorable carbon sources for the production of bioabsorbent from Alcaligenes latus B-16. Yeast extract at a concentration of 0.5% was also an effective medium for growth. Biopolymers from A. latus B-16 were purified, and the biopolymers were separated into two types of biopolymers : a high-molecular weight component (acid-biopolymer) and a low-molecular weight component (neutral-biopolymer). These two types of biopolymers were shown to be homogeneous on electrophoresis and GPC (Gel Permeation Chromatography on HPLC). The high-molecular weight component was bioabsorbent, and was able to absorb water at more than 1,000 times its own weight. The total yield of acid- and neutral-biopolymers from A. latus B-16 was 20-25g (dry weight) per liter of culture broth, and they were produced at a ratio of 20 : 1 (acid : neutral).
- 社団法人日本生物工学会の論文
- 1994-04-25
著者
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KURANE Ryuichiro
National Institute of Bioscience and Human-Technology (NIBH)
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Nohata Yasuhiro
Yokkaichi Laboratory Hakuto Co.ltd.
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Nohata Yasuhiro
Yokkaichi Laboratory Hakuto Co. Ltd.
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Kurane Ryuichiro
National Institute Of Biosciecne And Human Technology Agency Of Industrial Science And Technology Ministry Of International Trade & Industry
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