cDNA Cloning and Gene Expression of Phenylalanine Ammonia-Lyase in Lithospermum erythrorhizon
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概要
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Two cDNA clones (LEPAL-1 and LEPAL-2) encoding phenylalanine ammonia-lyase were isolated from cell suspension cultures of Lithospermum erythrorhizon. Northern kinetic studies showed that LEPAL-1 mRNA contents markedly increased one day after inoculation of the cells into fresh medium, then decreased to the steady-state level. The course of mRNA accumulation paralleled that of PAL enzyme activity. The rapid induction of PAL activity seems to reflect the induction of dihydroechinofuran biosynthesis, while shikonin was produced at the steady-state level of PAL activity. The course of LEPAL-2 mRNA accumulation seemed to be similar to, but much lower than that of LEPAL-1. In the intact plant, both genes are expressed mainly in the root, the organ in which shikonin is exclusively produced and accumulated. Genomic Southern blot analyses showed that both genes are present in the genome as single copies.
- 社団法人日本農芸化学会の論文
- 1997-12-23
著者
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Yazaki K
Kyoto Univ. Uji Jpn
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Yazaki Kazufumi
Faculty Of Pharmaceutical Sciences Kyoto University
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Heide L
Eberhard‐karls‐univ. Tuebingen Tuebingen Deu
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Heide Lutz
Pharmazeutische Biologie Pharmazeutisches Institut Eberhard-karls-universitat Tubingen
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KATAOKA Mieko
Faculty of Pharmaceutical Sciences, Kyoto University
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HONDA Gisho
Faculty of Pharmaceutical Sciences, Kyoto University
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SEVERIN Klaus
Institut fur Phrmazeutische Biologie, Albert-Ludwigs- Universitat Freiburg, Schanzlestrasse
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HEIDE Lutz
Institut fur Phrmazeutische Biologie, Albert-Ludwigs- Universitat Freiburg, Schanzlestrasse
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Honda G
Department Of Pharmacognosy Graduate School Of Pharmaceutical Sciences Kyoto University
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Honda Gisho
Faculty Of Pharmaceutical Sciences Himeji Dokkyo University
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Severin Klaus
Institut Fur Phrmazeutische Biologie Albert-ludwigs- Universitat Freiburg Schanzlestrasse
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Kataoka Mieko
Faculty Of Pharmaceutical Sciences Kyoto University
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