49. アラビドプシスPSK受容体の解析

概要

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AtPSKR1 encodes a 1008-amino-acid RLK that contains extracellular LRR, a 36-amino-acid island domain and a cytoplasmic kinase domain. Biochemical analysis of AtPSKR1 revealed that this receptor kinase interacts with [^3H]PSK with binding affinity of K_d=7.7 nM. We identified a knockout mutant of AtPSKR1 (pskr1-1) and confirmed the absence of specific [^3H]PSK binding activity in the membranes. Although no obvious phenotypic differences in plant growth were found between pskr1-1 and WT plants in vitro, most striking phenotype of the pskr1-1 plants was that their potential to dedifferentiate to callus was rapidly lost in age-dependent manner and that cell death was induced in the undivided cells of the leaf disks. Once cell division proceeded, callus derived from pskr1-1 leaf disks proliferated normally and eventually re-differentiated into shoots and roots irrespective of the age of the original tissues, suggesting that PSK regulates the signaling cascades related to the post-mitotic aging and cell death.
植物化学調節学会の論文
2004-10-13