C-31 ニンジン細胞培養系におけるペプチド性細胞増殖因子(PSK)の効果

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It is well known that proliferation of plant cells in culture is promoted by addition of conditioned medium(CM) to a fresh medium. Recently, a peptide plant growth factor, phytosulfokine-α(PSK-α), has been isolated from CM of asparaqus mesophyl cell cultures. However, there is no information on the physiological roles of PSKs. To clarify effects of PSKs on plant morphogenesis, we examined it using carrot cell cultures. When PSK-α was added to the induction medium of carrot somatic embryogenesis, the number of somatic embryos formed increased depending on the concentration of PSK-α, and the cell numbers also increased throughout the culture period by addition of PSK-α. 【1-4】PSK-α and 【1-3】PSK-α showed similar effects. On the other hand, addition of Tyr-SO_3H and 【2-5】PSK-α, negative control chemicals, showed no effect on somatic embryogenesis nor cell proliferation. When embryogenic cells(EC) were cultured in the medium containing 2, 4-D and PSKs, proliferation of EC was stimulated depending on the concentration of PSK-α, 【1-4】PSK-α and 【1-3】PSK-α. When non-embryogenic cells (NC), Which lost the ability to form somatic embryo, was used, cell proliferation in the medium containing 2, 4-D(1mg/L) was not affected by PSK-α, 【1-4】PSK-α, 【1-3】PSK-α and negative contol chemicals, Tyr-SO_3H and 【2-5】PSK-α but those in the medium containing low concentrations of 2, 4-D stimulated by addition of PSK-α. While in 2, 4-D deleted medium, cell proliferation of NC was not stimulated by PSK-α. These results suggest that PSKs stimulates carrot somatic embryogesis though promotion of cell division and PSKs may support the fuction of auxin, 2, 4-D.
植物化学調節学会の論文
1998-10-01

C-31 ニンジン細胞培養系におけるペプチド性細胞増殖因子(PSK)の効果

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