C-30 PSK-αのニンジン細胞分裂に及ぼす効果

概要

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PSK-α is a plant peptidal growth factor isolated from conditioned medium of Asparagus mesophyl cell culture. PSK-α is a modified pentapeptide whose tyrosine residues are sulfated. The apply of PSK-α to Asparagus mesophyll cell culture is known to result in activation of cell division at low cell density. PSK-α was found in vaious plant cell culture systems not only in monocotyledonous but also in dicotyledonous plants. These observations indicate that PSK-α is an indispensable factor in cell division of higher plants. In this study, we examined the effect of PSK-α on cell division cycle using synchronous culture of carrot non-embryogenic cells(NC). Synchronized cell division of NC was established by aphidicolin treatment. In this system, the first peak of cell division was observed 13-14 hours after elimination of aphidicolin, even though NC was precultured in MS medium with or without 2, 4-D (1mg/l). When PSK-α(1x10^-7M) was treated before or after aphidicolin treatment, mitotic index (MI) increased in both cases. However, the time after aphidicolin elimination showing maximum MI was the same in both cases and equivalent to that in negative control (no PSK-α treatment). These results may indicate that PSK-α functions in G1 or G0 phase but not in G2 or M phase and that PSK-α acts very rapid after the application.
植物化学調節学会の論文
1998-10-01