24 西洋わさび毛状根からの光誘導不定芽形成におけるサイトカイニンに対する反応性

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Infection with Agrobacterium rhizogenes causes formation of hairy roots aroung the infected area of plants. Hairy roots of horseradish (Amoracia rusticana) grew vigorously as roots when they were cultured on phytohomone-free MS medium in darkness. On the other hand, they formed adventitious shoots on the same medium in light. This light-induced formation of adventitious shoots was also observed on non-transformed roots and leaves which were cultured on phytohormone-free MS medium in light. Furthermore, it had been clarified that cytokinin involves in the light-induced formation of adventitous shoots. Frequency of light-induced formation of adventitious shoots was highest at the proximal end of hairy roots and increased with the increased duration of culture in darkness. Frequency of cytokinin-induced formation of adventitious shoots was also highest at the proximal end of hairy roots and increased with the increased duration of culture in darkness.These results indicate the some componenets involved in response to cytokinin may affect the difference of frequency of adventitious shoot formation induced by light or cytokinin in horseradish hairy roots.In the next experiment, we tried to isolate CKI1 homologue from horseradish. CKI1 gene isolated from Arabidopsis thaliana involves in cytokinin signal transduction pathway and may play as the cytokinin receptor in the two-component system. At fist, cDNA library from hairy roots and leaves were screened with CKI1 fragments from A. thaliana, but no positive clones were isolated. In the second, genomic library were screened and positive clones were isolated. Some of these clones showed similarity to CKI1 and a few copies of CKI1 homologue may present in horseradish genome.
植物化学調節学会の論文
1999-10-01

24 西洋わさび毛状根からの光誘導不定芽形成におけるサイトカイニンに対する反応性

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