47 ペブチド性植物細胞増殖因子PSKの植物個体における機能解析

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Phytosulfotdne (PSK) is the peptidyl plant growth factor and found in the culture medium from many dicot- and monocot- plants. But the function of PSK in whole plant is little known. In order to clarify the function of PSK in Arabidopsis thaliana ecotype Columbia plant, we investigated the effect of exogenously applied PSK to whole plant. Arabidopsis was cultured on Murashige and Skoog's (MS) medium containing PSK. On the mecdium containing PSK, the number of root tips increased significantly. The length of main root was longer than that of control at early stage (7 days after sowing), but not differentr at later stage (13 and 18 days after sowing). These results indicate that PSK enhances root growth and/or root branching. Expression analysis of PSK prectmsor genes was performed by RT-PCR. AtPSK3 was found to express in most organs examined, especially in flower stalk, flower, and silique. Expression of AtPSK2 was weak and limited in flower stalk. From these results, AtPSK3 may be a main PSK precursor gene in Arabidopsis. FXp. ression profile of AtPSK3 indicates that PSK has a role for stimulation of cell division in whole plant as same as in cultured cells, and might have another function in flower stalk, rosette leaf and mature seed. The present work indicates that PSK has a function not only in cultured cells but also in whole plant.
植物化学調節学会の論文
2001-10-09