33. PSK がニンジン胚軸の形質転換効率に与える影響

概要

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Relative growth rate of plant cells in vitro is considerably affected by the initial cell densities. This troublesome effect has been interfered with the establishment of an efficient plant cell culture system, especially in cases where only a small number of surviving cells are expected such as genetically transformed cells under the antibiotic selection. To promote the growth of these cells, some workers have used "nurse culture systems" such as a feeder layer, in which the feeder cells produce growth factor(s) inducing the division of target cells. Now the active principle involved in the density effect was identified as PSK, a small sulfated peptide easily prepared by chemical synthesis. Here we report that PSK significantly improves the efficiency of genetic transformation of carrot hypocotyl cells by supporting the growth of transformed cells. Chemical nursing using PSK might present a powerful tool for basic research in plant biology and biotechnological applications.
植物化学調節学会の論文
2003-10-10