44 Phytosulfokine(PSK)-αと2, 4-Dがニンジン培養細胞の細胞周期に及ぼす効果

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Phytosulfokine-α is the first isolated plant peptide growth factor which is synthesized and acts in the presence of auxin such as 2, 4-dichlorophenoxyacetic acid(2, 4-D), but the mechanisum of PSK action in cell cycle stimulation and relationship between PSK and 2, 4-D are still unknown. We established treatment and tested the effect of PSK and 2, 4-D on cell cycle. Carrot NC was pre-cultured in Murashige and Skoog's medium without 2, 4-D for two weeks. The treatment with PSK-α(1x10^-7M) under 2, 4-D-free condition raised the value of Mitotic index (MI), but did not affect the progress of cell cycle after S phase. The treatment with 2, 4-D(4.5x10^-6M) under PSK-free condition accelarated the cell cycle progress followed the increase of MI value. Furthermore, simultaneous application of PSK-α(1x10^-7M) and 2, 4-D(4.5x10^-6M) stimulated much more the cell cycle progress. The pattern of the accumulation of the transcripts of cyclin A and B also supported those observations. These results indicate that PSK-α acts in several stages of cell cycle and 2, 4-D is required as auxin for the action of PSK-α in S or G2 phase.
植物化学調節学会の論文
1999-10-01