45 タバコ花粉の発芽に対するファイトスルフォカインの効果

概要

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Density-dependent pollen germination and tube growth in vitro is a well-documented phenomenon, termed pollen population effect, but far less are known about its molecular basis. We present evidence to support phytosulfokine-α [Y(SO3H)IY(SO3H)TQ, PSK-α] is a native bioactive factor contributing to such effect. Mature pollen grains of Nicotiana tabacum var macrophylla were suspended in the medium mainly containing sucrose, PEG6000, CaCl2, KCl and H3BO3, and incubated at 25℃ under darkness for 2h. Pollen germination frequency increased in a density-dependent manner from 625 to 46000 grains/mL. Conditioned medium obtained from the medium cultured at the density of 10000 pollen grains/mL for 12h promoted pollen germination cultured at the low density (625 grains/mL). A rabbit antiserum against PSK-α specifically inhibited the promotive effect of the conditionaed medium. HPLC-ELISA quantification showed that the conditionaed medium contained 0.4 nM of SK-α. Exogenous PSK-α also stimulated pollen germination of the low density culture. PSK-α analogs, [2-5]PSK-α and (OH)2PSK-α which had not shown activity to other systems, had also no effect to the pollen germination. These results indicated that PSK-α is an important regulato involved in pollen population effect.
植物化学調節学会の論文
1999-10-01