Development of Microsatellite Markers in Melon ( Cucumis melo L. ) and Their Application to Major Cucurbit Crops

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概要

• 論文の詳細を見る

• We developed 31 sets of melon ( Cucumis melo L. ) microsatellite markers in this study. Primers for these markers were designed from clones containing simple sequence repeats ( SSRs ) of ( GA/CT )n or ( GT/CA )n isolated from a melon genomic library. These microsatellite loci were examined for the detection of alleles among twelve lines and cultivars from six varieties of C. melo. A total of 28 marker loci showed polymorphism by 2.0% agarose TBE gel separation. And these markers were also examined for cross-species amplification applicable to nine other species in Cucurbitaceae. Among them, 13 marker loci were commonly detected within the major cucurbit crops, cucumber, pumpkin and watermelon using the present primer sets. The species to which the melon microsatellite markers would be most applicable was Momordica charantia ( 24 markers ), followed by Cucumis sativus ( 20 markers ) and Cucurbita maxima ( 18 markers ). These conserved microsatellite loci could be used as anchor markers in studies on synteny in Cucurbitaceae.

• 日本育種学会の論文

著者

• Suwabe Keita

  National Institute of Vegetable and Tea Science

• Suwabe K

  National Inst. Vegetable And Tea Sci. Mie Jpn

• Suwabe Keita

  National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization

• Chiba Naoki

  National Institute Of Vegetable And Tea Science National Agricultural Research Organization : Presen

• Nunome Tsukasa

  National institute of Vegetable and Tea Science, National Agricultural Research Organization

• Hirai Masashi

  National institute of Vegetable and Tea Science, National Agricultural Research Organization

• Hirai M

  National Research Institute Of Vegetables Ornamental Plants & Tea(nivot)

• Hirai M

  Kyoto Prefectural Univ. Kyoto Jpn

• Hirai Masashi

  Faculty Of Agriculture Kyoto Prefectural University

• Nunome T

  National Inst. Vegetable And Tea Sci. Mie Jpn

• Nunome Tsukasa

  Natl. Res. Inst. Veg. Ornam Plant & Tea.

• Nunome Tsukasa

  Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv

• Nunome Tsukasa

  National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization

• Nunome Tsukasa

  National Institute Of Vegetable And Tea Science (nivts)

• Hirai Masashi

  National Institute Of Vegetable And Tea Science (nivts):(present Address) Faculty Of Agriculture Kyo

• Nunome Tsukasa

  National Institute Of Vegetable And Tea Science

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