Characterization of Trinucleotide Microsatellites in Eggplant
スポンサーリンク
概要
- 論文の詳細を見る
A small insert genomic library of eggplant ( Solanuum melongena L. ) was screened with eight trinucleotide probes. Based on the screening of approximately 108,000 plaques, the occurrence of (AAC/TTG)_n, (AAG/TTC)_n, (ACC/TGG)_n, (ACG/TGC)_n, (ACT/TGA)_n, (AGC/TCG)_n, (AGG/TCC)_n and (ATC/TAG)_n repeats was estimated to be, respectively, 1.2 x 10^3, 1.3 x 10^2, 4.0 x 10^2, 7.6 x 10, 1.1 x 10^2, 5.7 x 10, 1.3 x 10^2 and 3.8 x 10, i. e. once every 951 kbp, 8.3 Mbp, 2.8 Mbp, 14.5Mbp, 9.7 Mbp, 19.3 Mbp, 8.3 Mbp and 29 Mbp in the eggplant genome, respectively. Two trinucleotide repeats, (AAC/TTG)_n and (ACC/TGG)_n, predominated, accounting for 84.5% of the isolated trinucleotide microsatellites. A total of 83.5% of the identified trinucleotide repeats contained seven repeat units or less. Inserts of the positive clones were sequenced and 85 PCR primer sequences bordering the microsatellite repeats were designed. In order to assess the polymorphism, 11S. melongena lines and 11 Solanum relatives were used. The mean number of alleles per polymorphic locus was 2.1 for S. melongena lines and 2.9 for Solanum relatives. Low levels of polymorphism were observed in eggplant, with the average heterozygosity being 0.31 between S. melongena lines and 0.32 between Solanum relatives. Most of the markers that detected polymorphism between S. melongena lines contained eight or more repeat units. The motif most frequently found in Solanum was (AAC/TTG)_n in this study. All the primer sets which detected PCR products in S. melongena yielded PCR products in S. incanum, which is in agreement with its very close relationship with S. melongena. Further transferability, however, was rather low, since only half of the primer sets gave products in Solanum relatives except for S. incanum. Analysis of the polymorphic loci in an eggplant F_2 population revealed a codominant mendelian segregation.
- 日本育種学会の論文
著者
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Suwabe Keita
National Institute of Vegetable and Tea Science
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Suwabe K
National Inst. Vegetable And Tea Sci. Mie Jpn
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Suwabe Keita
National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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Nunome Tsukasa
National institute of Vegetable and Tea Science, National Agricultural Research Organization
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Nunome T
National Inst. Vegetable And Tea Sci. Mie Jpn
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Nunome Tsukasa
Natl. Res. Inst. Veg. Ornam Plant & Tea.
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Nunome Tsukasa
Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv
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Nunome Tsukasa
National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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Nunome Tsukasa
National Institute Of Vegetable And Tea Science (nivts)
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OHYAMA Akio
National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
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FUKUOKA Hiroyuki
National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
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Ohyama A
National Res. Inst. Vegetables Ornamental Plants And Tea Mie Jpn
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Ohyama Akio
Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv
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Ohyama Akio
Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.
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Fukuoka H
Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv
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Fukuoka Hiroyuki
National Inst. Of Vegetable And Tea Sci.
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Nunome Tsukasa
National Institute Of Vegetable And Tea Science
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Ohyama Akio
National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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