Suppression of Acid Invertase Activity by Antisense RNA Modifies the Sugar Composition of Tomato Fruit : GENE STRUCTURE AND EXPRESSION : PROTEINS, ENZYMES AND METABOLISM

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概要

• 論文の詳細を見る

• cDNA for an acid invertase (EC 3.2.1.26) of tomato (Lycopersicon esculentum Mill.) fruit was introduced into tomato plants under the control of the cauliflower mosaic virus 35S promoter in the antisense orientation. The antisense gene effectively suppressed the invertase activity in soluble and cell wall fractions from ripening fruits. The sucrose content of fruits of the transformants was markedly increased, while the hexose content was reduced. These results indicate that acid invertase is one of main determinants of the sugar composition of tomato fruit. The invertase activity in the cell wall fraction of the leaf tissues of the transformants was not suppressed to the same extent as that in the soluble fraction. Wounding of the control leaf tissues induced invertase activity in both soluble and cell wall fractions. The induction of activity in the soluble fraction was sup-pressed by the antisense gene, while that in the cell wall fraction was unaffected. These findings suggest that mRNA for some other invertase, in particular, the mRNA for a cell wall-bound invertase, was present in leaves.

• 日本植物生理学会の論文

著者

• Hirai Masashi

  National institute of Vegetable and Tea Science, National Agricultural Research Organization

• Hirai M

  National Res. Inst. Vegetables Ornamental Plants And Tea Mie Jpn

• Hirai Masashi

  Natl. Res. Inst. Veg. Ornam Plant & Tea.

• Nishimura Shigeo

  National Research Institute Of Vegetables Ornamental Plants And Tea (nivot)

• Imai Tuyoshi

  Natl. Res. Inst. Veg. Ornam. Plant & Tea.

• OHYAMA Akio

  National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization

• Ohyama A

  National Res. Inst. Vegetables Ornamental Plants And Tea Mie Jpn

• Ohyama Akio

  Molecular Genetics And Physiology Research Team National Institute Of Vegetable And Tea Science (niv

• Ohyama Akio

  Plant Breeding And Genetics Research Laboratory Japan Tobacco Inc.

• SATO Takanori

  National Research Institute of Vegetable, Ornamental Plants and Tea

• ITO Hidekazu

  National Institute of Vegetable and Tea Science, National Agricultural Research Organization

• IMAI Tuyoshi

  National Research Institute of Vegetables, Ornamental Plants and Tea(NIVOT)

• Ito Hidekazu

  National Research Institute Of Vegetables Ornamental Plants And Tea(nivot)

• Ito Hidekazu

  National Institute Of Vegetable And Tea Science National Agricultural Research Organization

• Nishimura S

  National Research Institute Of Vegetables Ornamental Plants And Tea (nivot)

• Hirai Masashi

  National Institute Of Vegetable And Tea Science (nivts):(present Address) Faculty Of Agriculture Kyo

• Sato T

  National Research Institute Of Vegetables Ornamental Plants And Tea(nivot)

• Sato Takanori

  National Research Institute Of Vegetable Ornamental Plants And Tea

• Ohyama Akio

  National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization

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